Table 2.

Antibodies and dyes used for identifying macrophages in human lung digest (see Note 2)

Marker	Clone	Suggested fluorochromes	Working concentration (μg/mL)	Host species	Target species
Live/Dead dye (e.g. DAPI)			1 μg/mL
CD206	15–2	PE-Cy7	0.12 μg/mL	Mouse	Human
CD43	84–3C1	APC	0.5 μg/mL	Mouse	Human
CD45	HI30	BUV395	1 μL/test	Mouse	Human