Figure 3.

Acetylation-deficient p53^4KR preserves coronary vasculature and coronary flow reserve (CFR). A and B, Representative images of Isolectin B4 (IB4, green; DAPI stains the nuclei, blue)-stained frozen heart sections and quantification of the number of capillaries/100 nuclei in the indicated groups (n=4–5). Bar=50 μm. C and D, Representative immunoblots and quantitative analysis of HIF-1α, HO-1, Ang-1, VEGF, VCAM-1, and GAPDH in the indicated mouse hearts (n=6–8). HIF-1α, HO-1, and corresponding GAPDH were from the same membrane. Ang-1 and corresponding GAPDH were from the same membrane. VEGF and corresponding GAPDH were from the same membrane. VCAM-1 and corresponding GAPDH were from the same membrane. E, Representative images of co-staining of cardiomyocyte marker Troponin-T with HIF-1α in frozen heart sections. F, Representative pulsed-wave Doppler images of the proximal left coronary arteries and CFR of WT and p53^4KR mice subjected to either sham or TAC procedure for eight weeks. CFR was calculated as the ratio of hyperemic peak diastolic flow velocity (2.5% isoflurane) to baseline peak diastolic flow velocity (1% isoflurane) in the indicated groups (n=6–8).