FIG. 2.
Comparison of the virus production upon infection of BHK-21 cell monolayers with (A) FMDV C-S8c1 and the MARLS variant (A) and chimeric FMDVs O1K/C-S8c1, O1K/MARLS, and O1K/MARLST2363, including the capsid of C-S8c1 or its hypervirulent derivatives (described in Materials and Methods) (B). In all cases, BHK-21 cells were infected at a multiplicity of infection of 5 PFU per cell. Virus titers at different times after infection were determined by plaque assays on BHK-21 cells monolayers (12). Each value represents the mean and standard deviation from triplicate assays.