TABLE 1.
Synthetic oligonucleotides used for retrotranscription and amplification of FMDV genomes
| Fragmenta | Oligonucleotide sequence (5′ to 3′) | Orientation |
|---|---|---|
| F1 (1–367) | TTGAAAGGGGGCGCTAGGGTC | Sense |
| GTTAACTGAAAGGCGGGTTTCGGGTG | Antisense | |
| F2 (368–973) | TAAGTTTTACCGTCTGTCCCG | Sense |
| AAACCGAGCGCTTTTATAG | Antisense | |
| F3 (569–1643) | CACGATCTAAGCAGGTTTCC | Sense |
| CCCTTGAGCTTTCGCT | Antisense | |
| F4 (1002–4189) | CGGAGGTCGGCACCTTTCCTTTAC | Sense |
| CAAACGTGCTGTCCAGAATCTC | Antisense | |
| F5 (3988–5699) | TTGGTGTCTGCTTTTGAGGAAC | Sense |
| GCCTTCTGACCTGGAAGAGTTC | Antisense | |
| F6 (5344–7156) | AAAGGCCAACACGAGGCAGC | Sense |
| CGTCGACAATGCGAGTCTTGCCG | Antisense | |
| F7 (6610–8115) | GGGTTGATCGTTGATACCAGAGA | Sense |
| GGATTAAGGAAGCCGGGAAAGCCC | Antisense | |
| C1 (3573–4189) | ACACCGTGTGTTGGCTACGGCG | Sense |
| CAAACGTGCTGTCCAGAATCTC | Antisense | |
| C2 (2817–4189) | CTTGGCCGGTTTGGCCCAGTAC | Sense |
| CAAACGTGCTGTCCAGAATCTC | Antisense | |
| C3 (1009–3534) | CGGCACCTTTCCTTTTATAACC | Sense |
| CTTGTGGTAGGCAGTGGGATTGGTT GTGTTGTTAAGTG | Antisense |
a
Amplified fragments of FMDV serotype C and chimeric type O-type C genomes are designated by F and C, respectively; numbers in parentheses indicate the oligonucleotide positions in the C-S8c1 genome, numbered as described in Materials and Methods (20).