TABLE 4.
Mutations and corresponding amino acid substitutions found in the FMDV MARLS genome as compared to FMDV C-S8c1
| Genomic region | Mutationa | Amino acid substitutionb | Secondary structurec |
|---|---|---|---|
| 5′ nontranslated region | C-247 → T | NCR | |
| C-338 → T | NCR | ||
| G-476 → A | NCR | ||
| T-518 → C | NCR | ||
| L protease | G-1091 → T | Arg-18 → Ile | |
| C-1105 → T | Pro-23 → Ser | ||
| A-1532 → G | Glu-165 → Gly | ||
| VP2 | G-2285 → A | Gly-130 → Asp | E-F loop |
| VP3 | T-2622 → C | − | |
| C-2624 → T | Ala-25 → Val | N terminus | |
| C-2826 → T | − | ||
| G-3067 → A | Glu-173 → Lys | G-H loop | |
| T-3082 → A | Cys-178 → Ser | G-H loop | |
| C-3202 → A | Gln-218 → Lys | C terminus | |
| VP1 | A-3328 → G | Lys-41 → Glu | βB strand |
| C-3423 → T | − | ||
| T-3638 → C | Leu-144 → Ser | G-H loop, antigenic site A | |
| A-3797 → G | His-197 → Arg | C terminus, antigenic site C | |
| C-3806 → A | Pro-200 → Gln | C terminus, antigenic site C | |
| 2B | A-4036 → G | Thr-52 → Ala | |
| 2C | G-4583 → A | Ser-80 → Asn | |
| A-5110 → G | Thr-256 → Ala | ||
| G-5133 → C | Gln-263 → His | ||
| A-5191 → G | Met-283 → Val | ||
| 3A | A-5364 → C | − | |
| 3C | T-6225 → C | − | |
| A-6295 → C | Met-109 → Leu | ||
| 3D | T-6744 → C | − | |
| A-7305 → C | Gln-232 → His | ||
| G-7554 → T | − | ||
| T-7564 → C | − | ||
| T-7932 → C | − |
a
Procedures for sequencing the entire FMDV genome are described in Materials and Methods. The first letter corresponds to the nucleotide found in the parental FMDV C-S8c1, and the number gives the nucleotide position in the C-S8c1 genome, according to reference 20. Mutation T-3638 → C (Leu-144 → Ser) is responsible for resistance to neutralization by MAb SD6 (37). Except for this mutation, the capsid coding sequence of C-S8c1 p213 is indistinguishable from that of MARLS.
b
The first amino acid is the one found in C-S8c1; amino acid residues are numbered independently for each protein. NCR, noncoding region; −, silent mutation.