TABLE 5.

Amino acid substitutions found in capsid proteins of FMDV variants with different passage histories in BHK-21 cell cultures^a

Viral protein	Positionb	Amino acid in:
		C-S8c1	C-S8c1 p100c10	MARLS	R100
VP2	130	Gly	−c	Asp	−
	192	Ala	−	−	Thr
	193	Gly	−	−	Ser
VP3	7	Cys	−	−	Val
	9	Asp	−	−	Ala
	13	Asn	−	−	His
	14	Met	−	−	Leu
	25	Ala	Val	Val	−
	173	Glu	Lys	Lys	−
	178	Cys	−	Ser	−
	194	Asp	−	−	−
	218	Gln	Lys	Lys	−
VP1	41	Lys	Glu	Glu	−
	46	Asp	Glu	−	−
	83	Thr	−	−	Ala
	144	Leu	−	Ser	−
	145	Ala	−	−	−
	148	Thr	−	−	Lys
	164	Val	−	−	−
	194	Gly	−	−	Asp
	197	His	Arg	Arg	−
	200	Pro	−	Gln	−

^aAll variant FMDVs compared are derived from clone C-S8c1, as depicted in Fig. 1 and as described in Materials and Methods. 

^bAmino acid residues are numbered independently for each protein, and the positions refer to clone C-S8c1 (20). No mutations were found in the VP4 coding region. 

^c−, no substitution found in the virus.