TABLE 2.
Treatment | No. of LCLs generated | No. of LCLs positive for EBV EBNA-2/no. of LCLs analyzed | No. of LCLs positive for baboon LCV EBNA-1/no. of LCLs analyzed |
---|---|---|---|
Control medium | 28 | 12/12 | 0/12 |
Baboon LCV | 51 | 43/43 | 33/43 |
A total of 107 T-cell-depleted human PBMCs were exposed to control medium or baboon LCV and cultured in a 96-well microtiter plate. Subsequent LCLs were analyzed for the presence of EBV DNA and baboon LCV DNA by amplification for EBV EBNA-2 and LCV EBNA-1 sequences, respectively (Fig. 5).