Table 1.

Treatment methods used in the dose setting test and main study

	Treatment method	S9 mix	Treatment group	Preparation amount (mL)			Dispense volume (mL/well)
				Culture medium	S9 mix	Negative or positive control, or NPP
Dose setting test	Short time treatment	–	Negative control (saline)	2.7	-	0.3	2
			NPP	2.7		0.3	2
		+	Negative control (saline)	2.2	0.5	0.3	2
			NPP	2.2		0.3	2
	Continuous treatment	–	Negative control (saline)	2.7	-	0.3	2
			NPP	2.7		0.3	2
Main study	Short time treatment	–	Negative control (saline)	11.7	-	1.3	5
			NPP	11.7		1.3	5
			Positive control	12.87		0.13	5
		+	Negative control (saline)	9.53	2.17	1.3	5
			NPP	9.53		1.3	5
			Positive control	10.70		0.13	5
	Continuous treatment	–	Negative control (saline)	11.7	-	1.3	5
			NPP	11.7		1.3	5
			Positive control	12.87		0.13	2

NPP, no-pain pharmacopuncture (four-herb extract consisting of Corydalis tube, Chaenomelis Fructus, Paeoniae Radix, and Glycyrrhizae Radix et rhizoma).