Figure 2.

Novel AAV variants transduce hSCs with high efficiency and specificity

(A) Representative images of pure cultured hSCs transduced with EGFP reporter AAVs packaged using indicated capsids at 1,000 vg/cell. Blue, DAPI; purple, S100 (SCs marker); green, AAV-encoded EGFP. Arrows show EGFP⁺/S100⁻ cells. Scale bar, 50 μm. (B) Percentage of EGFP⁺ SCs. Quantification was performed using ≥80 cells per image, and ≥3 images per variant. p values were determined by one-way ANOVA with Holm-Śidák’s multiple comparison test (∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001). Data are shown as mean ± SEM. (C) Representative immunofluorescence images of mixed cultured hSCs transduced with EGFP reporter AAVs packaged using indicated capsids at 1,000 vg/cell. For (A) and (C), arrows indicate EGFP⁺/S100⁻ cells. Scale bar, 50 μm. (D and E) Percentage of (D) EGFP⁺/S100⁺ cells and (E) EGFP⁺/S100⁻ cells. Quantification was performed using ≥30 cells per image, and ≥4 images per variant. p values were determined by one-way ANOVA with Holm-Śidák multiple comparison test (∗p ≤ 0.05; ∗∗p ≤ 0.01). Data are shown as mean ± SEM. (F) Proportion of EGFP⁺ cells in the mixed hSC culture. Percentages of S100⁺ and S100⁻ cells among total EGFP⁺ cells were calculated. p values were determined by unpaired t-test (∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗∗p ≤ 0.0001).