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Immunopeptidomic discovery of a start-gain neoantigen. (A) Representative MS2 spectrum of ILLNFSTTTK. (B) Representation of codons in the mutated 5’UTR sequence in transcript ENST00000539214 containing the start-gain c.-61C>T (red), as well as the predicted neopeptide (second row). A wild-type uAUG is also highlighted (yellow). (C) Visualisation of TISRover output from the 5’UTR section in which each bar represents a TISRover score for a uAUG.
