. 2024 Mar 15;15:1376628. doi: 10.3389/fphys.2024.1376628

TABLE 2.

Oligonucleotide QPCR primers.

Gene ^a	Accession number ^b	Primer sequence (5′ → 3′)	Orientation	Product size (bp)
GLUT1	NM_205209	TCCTGATCAACCGCAATGAG	Forward	60
GLUT1	NM_205209	TGCCCCGGAGCTTCTTG	Reverse	60
GLUT3	NM_205511	TTGGGCGCTTCATTATTGG	Forward	68
GLUT3	NM_205511	CTCACTGATGTACATGGGAACAAAG	Reverse	68
GLUT6	XM_423637	CGCTTTGGACGTTGACATTG	Forward	62
GLUT6	XM_423637	CTGGATGACTCGGGATGAGAA	Reverse	62
GLUT8	NM_204375	GCTGCCTCAGCGTGACTTTT	Forward	58
GLUT8	NM_204375	AGGGTCCGCCCTTTTGTT	Reverse	58
GLUT10	XM_417383	AACGCAGAACAAAGATTCCTGAA	Forward	65
GLUT10	XM_417383	GTCATTCCACGTGCCAGCTT	Reverse	65
GLUT12	XM_419733	TTTGTGGACCTGTTTCGTTCAA	Forward	61
GLUT12	XM_419733	GCGTGAGCCCTACCAGCAT	Reverse	61
GK	AY256906	TGTTACATGGAGGAGATGCACAA	Forward	78
GK	AY256906	GCCCCACTCCGTGTTCAC	Reverse	78
HK1	NM_204101	TTATGTGGTGCCGGAATGG	Forward	61
HK1	NM_204101	GCTCTAAGCCTCTGTTCTCCCTAA	Reverse	61
HK2	NM_204212	AGAGCCCTCTGATGCCACAT	Forward	57
HK2	NM_204212	GGAGGTGTCCGGAGAAAGG	Reverse	57
HK3	XM_001231328	CACCGGAGAAACCTTGTGAGA	Forward	72
		CTGACTCGGCCATGAAGCA	Reverse	72
GYS1	AB090806	GCCTCAACGTCCGCAAGTT	Forward	55
GYS1	AB090806	TGGGCGTGCAGGTTCTG	Reverse	55
GYS2	NM_001406729	CATTGACAAGGAAGCAGGAGAGA	Forward	67
GYS2	NM_001406729	GTGTACAGATGCCCGTTCCA	Reverse	67
GBE1	XM_015298401	AAGAAAATGGAGTATGGGAAATGG	Forward	71
GBE1	XM_015298401	TGAGGCACAGGAGAAAAACCA	Reverse	71
AGL	XM-040677682	TTCTAGCGTTTGGTGGGACTCT	Forward	58
AGL	XM-040677682	CCCTGGCCAAGCAGGTT	Reverse	58
mtATP8	HM142824	CACACTTGCCGGAACGTACA	Forward	57
mtATP8	HM142824	GCCGTTTGCGTGGAGATT	Reverse	57
GSK3α	XM_416557	AAGGCACATCCATGGACTAAGG	Forward	59
GSK3α	XM_416557	GACCCGTACTCCTGAGGTGAAA	Reverse	59
GCN2	XM_040671975	TGCGTCCTCAGGGATTGACT	Forward	66
GCN2	XM_040671975	GGCACTTGACCCACAGATCA	Reverse	66
EIF2S1	NM_001006477	GGCCGTCGCCAGAATG	Forward	59
EIF2S1	NM_001006477	CCTCCGGAAACTTATGCTGGTA	Reverse	59
EIF4EBP1	XM_424384	CTCTCCGTGTGGGTGTGAATAC	Forward	56
EIF4EBP1	XM_424384	CCCCACAGCCCATCATCA	Reverse	56

^{^a}

AGL, glycogen debranching enzyme; EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1; EIF2S1, eukaryotic translation initiation factor 2 subunit alpha 1; GBE1, glycogen branching enzyme 1; GCN2, General Control Nonderepressible 2 or Eukaryotic Translation Initiation Factor 2 Alpha Kinase 4; GK, glucokinase; GLUT, glucose transporter; GSK3α, glycogen synthase kinase 3 alpha; GYS, glycogen synthase; HK, hexokinase; mtATP8, mitochondrial ATP, synthase F0 subunit 8.

^{^b}

Accession number refer to Genbank (NCBI).