Table 2.
Distribution of Shh transcripts in the neuronal population of the mouse brain
| Region | Nucleus | HuC/D | Gad67 | ChAT | nNOS | TH |
|---|---|---|---|---|---|---|
| Cortex | Layer I | 1+ | 1+ | 0+ | 1+ | n.d |
| Layer II/III | 1+–3+ | 1+–3+ | 0+ | 1+–2+ | n.d | |
| Layer IV | 1+–2+ | 1+ | 0+ | 1+ | n.d | |
| Layer V | 1+–3+ | 1+–3+ | 0+ | 1+–3+ | n.d | |
| Layer VIa | 1+–3+ | 1+–2+ | 0+ | 1+–3+ | n.d | |
| Layer VIb | 1+–3+ | 1+–2+ | 0+ | 1+ | n.d | |
| Hippocampus | CA1 | 1+–3+ | 1+–3+ | / | 1+ | n.d |
| CA2 | 1+–3+ | 1+–3+ | / | 1+ | n.d | |
| CA3 | 1+–3+ | 1+–3+ | / | 1+–3+ | n.d | |
| Dentate gyrus | ||||||
| Granul cell layer | 1+–3+ | / | / | 1+ | n.d | |
| Polymorph layer | 1+–2+ | 1+–2+ | / | 1+ | n.d | |
| Molecular layer | 1+–2+ | 1+–2+ | / | 1+ | n.d | |
| Striatum | Caudate putamen | 1+–3+ | 1+–3+ | 0+ | n.d | n.d |
| Pallidum | Globus pallidus | n.d | 1+–4+ | 0+ | n.d | n.d |
| Magnocellular preoptic nucleus | n.d | 1+–4+ | 1+–3+ | n.d | n.d | |
| Hypothalamus | Arcuate hypothalamic nucleus | |||||
| Dorsal part | 1+–3+ | 1+–3+ | 1+ | 1+–3+ | n.d | |
| Ventral part | 1+–3+ | 1+–3+ | 1+ | 1+–2+ | n.d | |
| Ventromedial hypothalamic nucleus | ||||||
| Dorsomedial part | 1+–3+ | 1+–3+ | 1+ | 1+–3+ | n.d | |
| Central part | 1+–3+ | 1+ | 1+ | 1+–3+ | n.d | |
| Ventromedial part | 1+–3+ | 1+ | / | 1+–3+ | n.d | |
| Dorsomedial hypothalamic nucleus | 1+–3+ | 1+–3+ | 1+ | 1+–3+ | n.d | |
| Posterior hypothalamic nucleus | 1+–3+ | 1+–3+ | 1+ | 1+ | n.d | |
| Lateral hypothalamic nucleus | ||||||
| Anterior | n.d | 1+–3+ | / | n.d | n.d | |
| Posterior | 1+–3+ | 1+–3+ | 0+ | 1+–3+ | n.d | |
| Posterior | 1+–3+ | 1+–3+ | 0+ | 1+–3+ | n.d | |
| Median eminence | 1+–2+ | 0+ | / | 0+ | n.d | |
| Tuberal nucleus | 1+–2+ | 1+–2+ | 0+ | 1+ | n.d | |
| Anterior hypothalamic nucleus | n.d | 1+–2+ | / | n.d | n.d | |
| Sub-paraventricular zone | n.d | 1+–3+ | / | n.d | n.d | |
| Paraventricular hypothalamic nucleus | n.d | 1+–2+ | / | n.d | n.d | |
| Suprachiasmatic nucleus | n.d | 1+ | / | n.d | n.d | |
| Optic chiasma | n.d | / | / | n.d | n.d | |
| Midbrain | Ventral tegmental area | n.d | n.d | n.d | n.d | 1+–3+ |
| Substantia nigra, pars compacta | n.d | n.d | n.d | n.d | 1+–3+ | |
| Substantia nigra, pars reticulata | n.d | n.d | n.d | n.d | / | |
| Pons | Mesencephalic trigeminal nucleus | n.d | 1 + | 4+ | 1+–3+ | n.d |
| Pontine central gray | n.d | 1+–3+ | 1+–3+ | 1+–3+ | n.d | |
| Motor trigeminal nucleus | n.d | 1+–2+ | 4+ | / | n.d | |
| Medulla | Facial nucleus | n.d | 1+–2+ | 4+ | 1+–3+ | n.d |
| Intermediate reticular nucleus | n.d | 1+–2+ | / | 1+–3+ | n.d | |
| Gigantocellular reticular nucleus | n.d | 1+–3+ | / | 1+–3+ | n.d | |
| Parapyramidal nucleus | n.d | 1+ | / | / | n.d | |
| Cerebellum | Granule cell layer | n.d | 1+ | / | 0+ | n.d |
| Purkinje cell layer | n.d | 4+ | / | / | n.d | |
| Molecular layer | n.d | 1+ | / | 1+ | n.d | |
| White matter | n.d | 1+ | / | / | n.d |
Density of Shh mRNA was estimated by smfISH from N = 3–4 animals. 4 + : highest density, > 20 dots/cell; 3 + : high density, 10–20 dots/cell; 2 + : moderate density, 6–9 dots/cell; 1 + : low density, 2–5 dots/cell; 0 + : undetectable, 0–1 dot/cell; /: not relevant; n.d.: not determined