Table 2.
Differential characteristics of strain ET2T and phylogenetically related species of the genus Microbacterium.
| Characteristic | ET2T | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
|---|---|---|---|---|---|---|---|---|
| DNA G+C content (mol%) | 69.4 | 69.8 | 70.1 | 70.4 | 66.5 | 69.0 | 71 | 73 |
| Colony color | From white to purple | Yellow | White/yellow | Ivory | Yellow | Light yellow | White | Yellow |
| Growth temperature (°C) | 25–55 W (30–37) | 10–44 (32) | 10–37 | 28 | 10–42 | 10–37 | 4–50 (28) | 9–37 |
| Growth pH | 6.0–9.0 (7.0) | 5.0–10.0 (7.0–8.0) | 6.0–9.5 | 7.5 | nd | 6.0–9.0 (7.0) | 5.0–10.0 | 6.0–11.0 (7.0) |
| 5% NaCl tolerance (w/v) | + | + | + | − | − | − | + | − |
| Assimilation of: Glucose |
+ | + | + | + | W | + | + | + |
| Sucrose | + | + | − | + | nd | nd | nd | nd |
| Galactose | + | + | − | + | nd | nd | W | nd |
| D-mannose | + | + | + | + | + | + | + | + |
| D-maltose | + | + | + | + | + | + | nd | + |
| D-Mannitol | + | + | + | nd | + | + | + | + |
| Trisodium citrate | − | nd | nd | nd | − | − | + | − |
| Starch | W | +/− a | − | nd | nd | nd | nd | − |
| Gelatin | − | nd | + | nd | nd | − | − | + |
| Acid from glucose | W | nd | W | + | W | − | + | nd |
| Sucrose | + | nd | − | + | + | − | − | nd |
| Galactose | + | nd | W | + | nd | − | W | nd |
| D-mannose | + | nd | + | + | + | − | − | nd |
| D-maltose | + | − | − | + | + | + | + | nd |
| Catalase | + | + | + | + | + | + | + | + |
| Nitrate reduction | − | − | + | nd | − | − | − | − |
| Cell-wall diamino acid | Orn | Lys | Lys | Orn | Orn | Orn | Orn | Lys |
| Cell-wall sugars | Rha, Fuc, Glc, Gal | Man, Rib, Gal, Rha, Ara | nd | Rha, Rib, Man, Gal | 6dTal, Man, Gal |
Rha, Gal | Rib, Glc, Gal |
Rha, Gal |
| Fatty acids (%) | Anteiso-C15:0 (35.35), iso-C16:0 (31.96), anteiso-C17:0 (22.78); iso-C15:0 (4.0), C16:0 (3.08), iso-C17:0 (1.80), iso-C14:0 (1.02) |
Anteiso-C17:0 (43.4), anteiso-C15:0 (27.1), iso-C17:0 (9.6), iso-C16:0 (9.5), iso-C15:0 (6.8), C16:0 (2.5), iso-C14:0 (0.2) |
Anteiso-C15 : 0 (52.0), anteiso-C17 : 0 (25.1), iso-C16 : 0 (9.7), iso-C15:0 (5.5), C16:0(3.5),iso-C17:0 (3.0), C17:0 (0.3), C18:0 (0.3), iso-C14:0 (0.3), iso-C18:0(0.2), anteiso-C15:0 (0.1) |
Anteiso-C15:0 (48.6), anteiso-C17:0 (24.4), iso-C16:0 (12.1), iso-C15:0 (7.0) |
Anteiso-C15:0 (55.0), anteiso-C17:0 (31.1), iso-C16:0 (11.3) |
Anteiso-C15:0 (48.1), anteiso- C17:0 (34.7-36.6 a ), iso-C16:0 (10.5- 12.5 a ), anteiso-C17:1 (2.1), C14:0 (1.1) |
Anteiso-C15:0 (38.5), C16:0 (18.9), anteiso-C17:0(17.1), iso-C16:0 (14.1), C18:0(6.0), C14:0 (2.8), iso-C14:0 (1.7), iso-C15:0 (1.0) |
Anteiso-15:0 (61.65), anteiso-C17:0 (26.22), iso-C16:0 (7.30), iso-C16 : 0 (3.09) |
Strain/species: strain ET2T (VKPM Ac-2212 = VKM Ас-2998); 1, M. kunmingense JXJ CY 27-2T; 2, M. invictum DC-200T; 3, М. сaowuchunii ST-M6T; 4, M. schleiferi JCM 9175T; 5, M. hatanonis JCM 14558T; 6, M. sediminis ylb-01T; 7, M. saccharophilum K-1T.
Data for ET2T were obtained in this study. Other data for the reference strains were taken from Kageyama et al. (2006); Bakir et al. (2008); Vaz-Moreira et al. (2009); Yu et al. (2013); Tian et al. (2021); Xiao et al. (2022), and Ohta et al. (2013);
Data were taken from Kageyama et al. (2006) and Yu et al. (2013).
+, positive; −, negative; W, weakly positive; nd, no data. Orn, ornithine; Lys, lysine; Ara, arabinose; Fuc, fucose; Gal, galactose; Glc, glucose; Man, mannose; Rha, rhamnose; Rib, ribose; 6dTal, 6-deoxy-l-talose.