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In vitro activation of Akt by polyomavirus middle T antigen. Akt was immunoprecipitated from lysates prepared from cells that had been starved for serum for 24 h. F111, normal rat fibroblasts; PyF, F111 cells transformed by wild-type virus; PyF-315YF, F111 cells transformed by 315YF virus; PyF-250YS, F111 cells transformed by 250YS virus. Data are averaged from two to seven experiments. Error bars show standard deviations.
