FIG. 6.

Serum starvation-induced apoptosis in Rat-1 clones expressing p53val135 along with the neomycin resistance vector alone (Neo [control]) or T antigens (wild type [WT], 315YF, or 250YS). Four plates of each cell type were grown in DMEM containing 10% calf serum at 38.5°C to 70% confluence. Two plates remained in 10% serum, and two plates were changed to serum-free medium. Cells in 10% serum were either fixed at 0 h (solid bars) or shifted to 32°C (vertically striped bars) and fixed at 48 h. Cells in 0% serum either remained at 38.5°C (hatched bars) or were shifted to 32°C (open bars), and then both were fixed at 48 h. Data are averaged from four to seven separate experiments. Error bars show the standard errors of the means.