Figure S4.

Supporting data for siRNA knockdowns of various PB components that lead to an increase in SGs, docking of smaller PBs, or loss of PB–SG docking. (A) Example immunoblots for the corresponding proteins after siRNA knockdowns. Fraction of the remaining protein is indicated (normalized to the corresponding GAPDH control) with errors representing the standard deviation of three biological replicates. (B) Quantification of other PB parameters related to Fig. 5 B. The blue box highlights conditions that lead to an increased number of PBs. (C) Quantification of other SG parameters related to Fig. 5. (D) IF images of EDC puncta (IF, magenta) and SGs (PABPC1, green) in DDX6 KO cells upon siRNA knockdown of PB proteins as in Fig. 5. (E) Quantification of the number of EDC4 spots per cell (right) and % docking interactions with SGs (left). The blue box highlights conditions that, in wild-type cells, lead to an increase in smaller PBs but show no effect in DDX6 KO cells. The green box highlights conditions that lead to reduced PB–SG docking. (G) Quantification of other SG parameters related to Fig. 5 D. All IF images are representative images of three independent biological replicates with more than three images analyzed per replicate. For quantification, data points from three biological replicates are shown in red-orange-pink, the mean replicate values are indicated as triangles, and mean values are shown at the bottom of the image (m). The number (n) of analyzed cells is shown at the top of the image. ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05, n.s. P > 0.05 (unpaired, two-tailed t test on individual data points). Scale bar, 10 μm if not indicated. The Non-Targeting Control siRNA Pool #2 was abbreviated with siNeg Control or siNeg2. Columns marked as “−” are untransfected cells. Source data are available for this figure: SourceData FS4.