Skip to main content
. 2024 Mar 29;10:35. doi: 10.1038/s41421-024-00665-0

Fig. 2. Long-term ingestion of microplastics disrupts gut microbiota and impairs hematopoietic system in mice.

Fig. 2

a Representative images of small intestine stained with hematoxylin-eosin (H&E). The image below (scale bar, 200 μm) is a magnification of the image above (scale bar, 500 μm). b Chiu’s score of intestinal section. c Representative images of Transmission Electron Microscope (TEM) staining in small intestine. The image below (scale bar, 1 μm) is a magnification of the image above (scale bar, 2 μm). White double headed arrow indicates microvillus length. d FITC-dextran uptake in vivo was measured in peripheral blood collected from ctrl, PSL and PSH mice to detect intestinal permeability. e Experimental schema of 16s rDNA sequencing from ctrl, PSL and PSH mouse feces (n = 6 per group). A detailed list of Operational taxonomic units (OTUs) list is provided in Supplementary Table S1. f Venn diagram of three groups. g, h PcoA plot showing microbial compositional differences between three groups (g) as quantified by UniFrac distance (h). i Schematic of 4-week fecal microbiota transplantation (FMT) (n = 6 per group). j Representative flow cytometry images. k, l Proportion (k) and absolute number (l) of cells in BM from recipient mice. m Percentage of apoptotic rate in HSCs. n, o Representative flow cytometry images of cell cycle (n) and proportion within LT-HSCs (o) in recipient mice. Error bars indicate SD, unpaired two-tailed t-test, *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant.