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. 2024 Mar 29;10:35. doi: 10.1038/s41421-024-00665-0

Fig. 5. HSCs defects in microplastic model are associated with defects in the Wnt signaling pathway.

Fig. 5

a Schematic of RNA-seq workflow (n = 3 per group). b PCA plot of BM LT-HSCs in ctrl and PSH group. c Expression of Hprt in LT-HSCs. d KEGG analysis of upregulated and downregulated signaling pathways in PSH mice. Red bars, downregulated in PSH; blue bars, upregulated in PSH. e Relative expression of genes in the Wnt signaling pathway. f Relative expression of Hprt in Rikenellaceae treatment or hypoxanthine treatment group (n = 3 per group). g Relative mRNA expression of genes within Rikenellaceae treatment group or hypoxanthine treatment group. h Enzyme-linked immunosorbent assay (ELISA) to detect WNT10A in BM supernatant within Rikenellaceae treatment group or hypoxanthine treatment group (n = 5 per group). i Schematic outlining BM cell analysis (n = 3 per group). j Absolute number of LT-HSCs. k Apoptosis levels in LT-HSCs. l, m Representative images of CFUs from 8-day cultured BM cells (scale bar, 500 μm) (l) and statistical data (m). Error bars indicate SD, unpaired two-tailed t-test. *P < 0.05, **P < 0.01, ***P < 0.001.