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. 2024 Mar 28;15:2727. doi: 10.1038/s41467-024-46882-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Subcellular localization of GFP::KIN-3 in wide type Day 1 and wild type Day 7 worms. n. Total number of animals scored. b The activity of CK2 to phosphorylate purified TOFU-4 peptide is decreased in the aging process. Lysates from wide type Day 1 and wild type Day 7 worms of adulthood were used in in vitro phosphorylation assays. The relative activity of CK2 from wide type Day 1worms is set to 1.0. Data is shown as mean ± SD. n = 3 independent biological replicates. Compared to wild type Day 1 worms, the nuclear foci of USTC factors GFP::TOFU-4 (c) and mCHERRY::PRDE-1 (d) in wild type Day 7 worms disappear. n. Total number of animals scored. e GFP::PRG-1 becomes diffused in wild type Day 7 worms compared to those of wild type Day 1 worms. n. Total number of animals scored. f Immunoblotting assays revealed that protein levels of GFP::PRG-1 are decreased in extracts of wild type Day 7 worms compared to those of wild type Day 1 worms. One representative out of 3 independent experiments is shown. g Bar diagram displaying piRNAs mapping perfectly to known C. elegans piRNA loci in wild type Day 1 and wild type Day 7 worms. h Bar diagram displaying the number of piRNA precursors mapping to piRNA sequences in wild type Day 1 and wild type Day 7 worms. Small RNAs are pretreated with 5’ Pyrophosphohydrolase (RppH, NEB). i Percentage of worms with expressed piRNA sensors in wide type Day 1 and wild type Day 7 worms. n. Total number of animals scored. j Western results of GFP::CSR-1 protein levels in wide type Day 1 and wild type Day 7 worms. One representative out of 3 independent experiments is shown. k qRT–PCR of turmoil1 and line2h transposon levels in wide type Day 1 and wild type Day 7 worms relative to act-3 mRNA are displayed. mRNA level in wild type worms is set to 1.0. Data is shown as mean ± SD. n = 4 independent biological replicates; p values from two-tailed Student’s t test. **p < 0.01. Scale bars: 20 μm (a and c–e), 50 μm for i and 10 μm (insets in a). Source data are provided as a Source Data file.