TABLE 2.

Surface expression of WT and mutant US28^a

MYC-tagged receptor	GFP-positive cells (%)	MYC-positive cellsb (%)
None (Rc/CMV vector)	24.1	0.3
WT US28	19.2	15.5
US28 Δ317	21.7	25
US28 K158V K159V	19.9	11.4
US28 K257V	24.8	9.9
US28 E266V RS267V	20.2	9.6

^aHeLa P4 cells were cotransfected in six-well trays with pCDNA3 expression vectors for different MYC-tagged forms of US28 and with EGFP-N1, a GFP expression vector (6:1 ratio). Cells were harvested 36 h later, stained with the anti-MYC monoclonal antibody 9E10 and with a PE-conjugated secondary antibody, and analyzed for GFP expression (green fluorescence) and MYC expression (red fluorescence). Results are from the same series of transfections. 

^bAmong GFP-positive cells.