Table 2.

Summary of crystal structures solved in this study

Enzyme	Substrate	Cofactor	Resolution	Treatment	PDB ID
PfuSAHH	SIH	NAD+	2.0 Å	22–25 °C	7R38
PfuSAHH	Inosine	NAD+	2.3 Å	22–25 °C	7R37
PfuSAHH	Inosine	NAD+	2.0 Å	95 °C, 15 min	8QNO
MmaSAHH	Inosine	NAD+	2.5 Å	22–25 °C	7R3A
SacSAHH	Adenosine	NAD+	2.6 Å	22–25 °C	7R39
MmSAHH	Inosine	NAD+	2.5 Å	22–25 °C	8COD

The molecules in the asymmetric unit in all the cases were almost identical, being superimposable with a root-mean-square deviation (r.m.s.d.) of 0.17–0.63 Å over 356–393 C_α atoms. Data collection and refinement statistics are summarised in Table 3.