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. 2024 Mar 28;15:2725. doi: 10.1038/s41467-024-47008-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Quantification of the BrdU-incorporation assay. N = 3 biological replicates ~10.000 cells were analyzed per condition in each replicate. Data are presented as mean values -/+ SD. Statistical significance was determined by one-way ANOVA + Dunnett’s multiple comparison test (****p ≤ 0.0001). Source data are provided in the Source Data file. b Same setup as in a, but comparing RPE1-hTERT-DAAO^H2B WT cells with p53KO cells. N = 3 biological replicates ~ 10.000 cells were analyzed in each replicate per condition. Data are presented as mean values -/+ SD. Statistical significance was determined by one-way ANOVA + Dunnett’s multiple comparison test (****p ≤ 0.0001). Source data are provided in the Source Data file. c Cell cycle profile analysis of RPE1-hTERT-DAAO^H2B and RPE1-hTERT-DAAO^TOM20 cells. d Quantification of (c), showing the percentage of cells with a 4 N DNA content. Dots represent 3 biological replicates ~ 10.000 cells were analyzed in each replicate per condition. Data are presented as mean values -/+ SD. Statistical significance was determined by one-way ANOVA test + Dunnett’s multiple comparison test (**p ≤ 0.01, ****p ≤ 0.0001, DAAO^H2B 2.5 mM D-Ala = 0.0367, 5 mM D-Ala & 10 mM D-Ala ≤ 0.0001). Source data are provided in the Source Data file. e Quantification of cell cycle profiles of nocodazole treated cells. Dots represent 3 biological replicates ~ 10.000 cells were analyzed in each replicate per condition. Data are presented as mean values -/+ SD. Statistical significance was determined by one-way ANOVA test + Dunnett’s multiple comparison test (*p ≤ 0.05, DAAO^H2B 2.5 mM D-Ala = 0.0399). Source data are provided in the Source Data file. f Quantification of cell cycle profiles of RPE1-hTERT-DAAO^H2B cells containing the FUCCI cell cycle indicator. Dots represent 3 biological replicates ~ 10.000 cells were analyzed per replicate per condition. Data are presented as mean -/+ SD. Statistical significance was determined by a two-sided unpaired t-test (**p ≤ 0.01, L-ala vs. D-Ala = 0.0097). Source data are provided in the Source Data file.