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Oufti detection parameters used in this study
| Parameter name | Value |
|---|---|
| cellDetection | |
| ThreshFactorM | 0.9925 |
| ThreshMinLevel | 0.8 |
| EdgeSigmaL | 1 |
| spotDetection | |
| minHeight | 0.002 |
| minWidth | 0.5 |
| maxWidth | 10 |
| Adjusted squared error | 0.4 |
| objectDetection | |
| Bkg subtraction method | 3 |
| Bkg subtraction threshold | 0.1 |
| Bkg filter size | 8 |
| Smoothing range (pixels) | 3 |
| LOG filter | 0.1 |
| Sigma of PSF | 1.5 |
| Fraction of object in cell | 0.4 |
| Minimum object area | 50 |
