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p47phoxbinds to WAVE1–Abi2–HSPC300 subcomplex of WRC.A, schematic showing WRC subcomplexes used in this study. B and C, Coomassie blue–stained SDS-PAGE gels showing glutathione-S-transferase (GST) pull-down results. B, GST-p47phox AB pulling down WRC indicated subcomplexes. C, GST-p47phox AB pulling down trimer in the presence of p22phox ICD peptide as competitors. ICD, intracellular domain; WRC, WAVE regulatory complex.
