FIG. 4.

Binding of macrophagetropic viruses to CCR5-negative Jurkat T cells upregulates expression of cytokine, chemokine, and FasL genes. The cells were incubated for 3 h at 37°C with sucrose gradient-purified Ba-L (A) or AD8 (B) virions. Total RNA was isolated, and mRNA expression was analyzed by RT-PCR using primers described in Materials and Methods. GAPDH served as an internal control. PCR products were resolved on 2% agarose gels and stained with ethidium bromide.