FIG. 7.

SDF-1 does not affect the HIV-1-stimulated expression of cytokine and chemokine genes in Jurkat T cells. The cells were left untreated (control) or were stimulated with sucrose gradient-purified NL4-3 (A) or Ba-L virions (B), SDF-1 (5 μg/ml), or a combination of HIV-1 and SDF-1. After 3 h at 37°C, total RNA was isolated and expression of cytokine and chemokine genes was analyzed by RT-PCR as described in the legend to Fig. 2. GAPDH served as an internal control.