Fig. 2.

In vitro and in vivo tumor toxicity of anti-calnexin antibody. (A) The dotted line illustrates concentration-dependent cytotoxicity, while the solid line represents complement-dependent cytotoxicity when the complement system is activated by adding goat serum to achieve a final concentration of 5% (vol/vol). Experiments were performed in duplicate at least three times. (B) Antitumor effect of anti-calnexin antibody. A549 cells were subcutaneously injected into backs of nude mice and treated intraperitoneally with 1 μg and 5 μg of anti-calnexin antibody on days 12 and 16 when the tumor size reached 70-150 mm³ after 10 days. *P-value < 0.05 (n = 9).