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. 2024 Feb 16;21(3):1125–1136. doi: 10.1021/acs.molpharmaceut.3c00671

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Biocompatibility of NPs in MCF-7 cells. (A) Viability of MCF-7 cells after exposure to PEG-MnO₂ NPs, 50:50 PLGA-MnO₂ NPs, 75:25 PLGA-MnO₂ NPs, and PLA-MnO₂ NPs in 2D culture measured using MTS assay. (B) Representative images of LIVE/DEAD stain on MCF-7 spheroids treated with 200 μg/mL Mn equivalent of NPs. (C) Quantification of LIVE/DEAD stain on MCF-7 spheroids to verify viability after incubation with different formulations of MnO₂ NPs. Comparisons were made between the control (0 μg/mL) group for each set of nanoparticles. Statistically significant treatment groups were denoted with *P < 0.001 and ^#P < 0.01.