Fig. 7.
Effects of Agent Orange Herbicidal Toxin Exposures on AD Biomarker Expression. Rat FLSCs treated for 24 h with Vehicle (Veh), 2,4-D, 2,4,5-T, or D + T were analyzed for A) Tau, B) pTau-pS396-Tau; PHF), C) AβPP, D) Aβ, E) ChAT, and F) AChE immunoreactivity by duplex ELISA with results normalized to RPLPO. Each group included 6 replicate cultures with 5 or 6 frontal lobe slices per well. Results were analyzed by one-way ANOVA with post hoc Tukey tests (repeated measures; See Table 5). Significant p-values are indicated within the graphs (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).