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Antibody and reporter cycle layout, including LED intensities and exposure times for each marker
| Cycle # | Cy3 (LED 100%) | Exposure time (ms) | Cy5 (LED 100%) | Exposure time (ms) | Cy7 (LED 60%) | Exposure time (ms) |
|---|---|---|---|---|---|---|
| 1 | BLANK | 250 | BLANK | 350 | BLANK | 500 |
| 2 | e-cadherin-RX014 | 750 | ERG-RX031 | 850 | SMA-RX013 | 500 |
| 3 | CD8-RX026 | 750 | CD3e-RX045 | 850 | PanCK-RX019 | 400 |
| 4 | Empty | 250 | p63-RX046 | 700 | Empty | 500 |
| 5 | BLANK | 250 | BLANK | 350 | BLANK | 500 |
