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Env incorporation into pseudotyped virus particles. Equal amounts of 293T cell viral supernatants were centrifuged through 20% sucrose as described in Materials and Methods. (A) Pellets were resuspended in sample buffer, boiled, analyzed on a Western blot probed with the anti-A tail antibody, and visualized by ECL. (B) The same blot was reprobed with the anti-Gag antibody and visualized by ECL.
