KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Chemicals | |||||||||
| Octopamine (OA) | Tocris | Cat #2242 | |||||||
| Epinastine (Ep) | Abcam | Cat #108929-04-0 | |||||||
| Acetylcholine (ACh) | Solarbio | Cat #G8320 | |||||||
| 5-hydroxytryptamine (5-HT) | Tocris | Cat #3547 | |||||||
| Histamine (HA) | PerkinElmer | Cat#NET732 | |||||||
| Glutamate (Glu) | Sigma-Aldrich | Cat #V900408 | |||||||
| γ-aminobutyric acid (GABA) | Tocris | Cat #0344 | |||||||
| Tyramine (TA) | Sigma-Aldrich | Cat #V900670 | |||||||
| Norepinephrine (NE) | Sigma-Aldrich | Cat #A9512 | |||||||
| Dopamine (DA) | Sigma-Aldrich | Cat #H8502 | |||||||
| All-trans-retinal | Sigma-Aldrich | Cat #R2500 | |||||||
| 3-Octanol (OCT) | Sigma-Aldrich | Cat #218405 | |||||||
| 4-Methylcyclohexanol (MCH) | Sigma-Aldrich | Cat #153095 | |||||||
| Mecamylamine (Meca) | Sigma-Aldrich | Cat #M9020 | |||||||
| Tiotropium bromide (Tio) | Dexinjia Bio & Tech | N/A | |||||||
| Deschloroclozapine (DCZ) | MedChemExpress | Cat #HY-42110 | |||||||
| Mineral oil | Sigma-Aldrich | Cat #69794 | |||||||
| Cell lines | |||||||||
| HEK293T | ATCC | Cat#CRL-3216; RRID: CVCL_0063 |
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| HTLA cells for Tango Assay | Gift from Bryan L. Roth | N/A | |||||||
| Recombinant constructs | |||||||||
| pDisplay-OA1.0-IRES-mCherry-CAAX | This study | N/A | |||||||
| pCMV-OA1.0 | This study | N/A | |||||||
| pCMV- Octβ2R -pHluorin | This study | N/A | |||||||
| pTango-Octβ2R | This study | N/A | |||||||
| pTango-OA1.0 | This study | N/A | |||||||
| pcDNA3.1-CAG-RFlamp | This study | N/A | |||||||
| Drosophila strains | |||||||||
| UAS-OA1.0 (chr2) | This study | N/A | |||||||
| LexAop2-OA1.0 (chr2) | This study | N/A | |||||||
| LexAop2-OA1.0 (chr3) | This study | N/A | |||||||
| 30y-GAL4 | Yi Rao, Peking University | BDSC: 30818 | |||||||
| Tdc2-GAL4 | Yi Rao, Peking University | BDSC: 9313 | |||||||
| MB247-LexA | Yi Zhong, Tsinghua University | N/A | |||||||
| R13F02-LexA | Yi Rao, Peking University | BDSC: 52460 | |||||||
| R13F02-GAL4 | Yi Rao, Peking University | BDSC: 48571 | |||||||
| UAS-TβH-RNAi | Jianquan Ni, TsingHua Fly Center | TH201500898.S | |||||||
| UAS-CsChrimson-mCherry | Bloomington Drosophila Stock Center | BDSC: 82180 | |||||||
| UAS-CsChrimson-mCherry | Bloomington Drosophila Stock Center | BDSC: 82181 | |||||||
| UAS-Kir2.1 | Chuan Zhou, Institute of Zoology, CAS | N/A | |||||||
| UAS-hM4Di | Donggen Luo, Peking University | N/A | |||||||
| Canton-S (W1118) | Yi Rao, Peking University | N/A | |||||||
| Trh01 (Trhn −/−) | Qian et al.[89] | ||||||||
| TβH mutant (TβHM18) | Liming Wang, Shenzhen Bay Laboratory | BDSC: 93999 | |||||||
| LexAop2-ACh3.0 | Jing et al.[45] | BDSC: 86551 | |||||||
| LexAop2-GCaMP6s | Bloomington Drosophila Stock Center | BDSC: 44274 | |||||||
| LexAop2-DA2m | Sun et al.[47] | BDSC: 90880 | |||||||
| UAS-Octβ1R-RNAi | Vienna Drosophila Resource Center | VDRC:110537 | |||||||
| TH-GAL4 | Yi Rao, Peking University | N/A | |||||||
| Software | |||||||||
| Origin 2019 | OriginLab |
http://www.originlab.com/; RRID:SCR_014212 |
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| ImageJ | NIH |
https://imagej.nih.gov/ij/; RRID: SCR_003070 |
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| Arduino Uno | Arduino.cc |
https://www.arduino.cc/en/Guide/ArduinoUno; RRID:SCR_017284 |
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| MATLAB R2019b | MathWorks |
https://www.mathworks.com/; RRID:SCR_001622 |
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