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[Preprint]. 2024 Mar 12:2024.02.22.581559. [Version 2] doi: 10.1101/2024.02.22.581559

Figure 3: Xist-mediated silencing of escapees leads to loss of TAD-like domains on the Xi.

Figure 3:

a-b, Capture Hi-C interactions and insulation score at the (a) Mecp2-Hcfc1 and (b) Kdm5c cluster in clone E6 prior to and upon doxycycline treatment. Capture Hi-C interactions are shown for the active (Xa) and the inactive (Xi) X chromosomes in untreated conditions (control no Dox) (top) and for the Xi upon doxycycline treatment for 7 days (middle) and 21 days (bottom) for the Mecp2-Hcfc1 cluster and 7 days for the Kdm5c cluster. Capture Hi-C data is shown at 10-kb resolution. Heatmaps showing allelic ratios for 29 (a) and 27 (b) X-linked genes included in the captured regions are shown. Escapees belonging to groups 6 (green) and 7 (pink) within Mecp2-Hcfc1 cluster are highlighted. c, Capture Hi-C interactions and insulation score at the Mecp2-Hcfc1 cluster for Xi in clone CL30.7. Left to right: untreated cells (control no Dox); 21 days of doxycycline induction; 23 days of auxin treatment in combination with 21 days of doxycycline induction. Capture Hi-C data is shown at 10-kb resolution. Heatmaps showing allelic ratios for 29 X-linked genes included in the captured region are shown. d, Capture Hi-C interactions and insulation score at the Kdm5c cluster for Xi in clone E6 after 7 days of doxycycline treatment followed by 4 days of doxycycline washout. The 27 X-linked genes included in the captured regions are shown.