Extended Data Fig. 4. Surface conservation and electrostatic property.

a-b. Overall surface conservation (a) and electrostatic potentials (b) of Retriever complexed with SNX17 CT peptide (shown in cartoon presentation). Surface conservation was calculated with ConSurf³³, with color-to-white gradients representing the most (ConSurf score = 9) to the least conserved residues (ConSurf score = 1) following the same color scheme used in Fig. 2c, d. CCDC22-CCDC93 VBD model derived from AlphaFold-Multimer predictions are shown as semi-transparent cartoon presentation as a reference. c. Cartoon presentation (left) and surface presentation of electrostatic potentials (right) of Retromer, showing the binding sites for indicated ligands.