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. 2024 Mar 29;10(13):eadk1200. doi: 10.1126/sciadv.adk1200

Fig. 7. AS-NP treatment prevents ConA-induced ALI.

Fig. 7.

(A) Survival analysis of C57BL/6J mice pretreated with free AS or AS-NPs (20 mg/kg) and then intraperitoneally injected with ConA (30 mg/kg). n = 6 per group. (B to I) C57BL/6J mice pretreated with free AS or AS-NPs (20 mg/kg) and then intraperitoneally injected with ConA (15 mg/kg) for 24 hours. n = 5 biologically independent mice. Serum levels of AST (B) and ALT (C). Images of H&E staining in liver cross sections (D). The mRNA expression of PTGS2 was evaluated by real-time PCR (E). Heatmap of all major PE/PC species in liver with hierarchical clustering of control, ConA, ConA + AS, and ConA + AS-NPs. Each PE/PC species was normalized to the corresponding mean value (F). The mRNA expression of IFN-γ (G) and IL-6 (H) in liver was evaluated by real-time PCR. IL-1β (I) in serum was analyzed by ELISA. Data are the mean ± SEM. Statistical analysis was performed using an unpaired two-tailed Student’s t test [(B), (C), (E), and (G) to (I)] or generalized Wilcoxon test (A). *P < 0.05, ***P < 0.001.