Figure 1.

Targeting the atp1 transcript with a modified RPF2 protein leads to delayed plant growth and curled leaf phenotype. A) RNA targets for RPF2 (in nad9 5′-UTR) and RPF2-atp1 (in atp1 coding sequence) and respective binding predictions. Dark green squares represent a perfect match, light green a partial match, white a neutral match, and magenta a mismatch according to the PPR code. The differences between RNA targets are highlighted by orange squares and the modifications in the protein by cyan squares. B) and C) show the phenotypes of 4 lines (T2 generation) transformed with the RPF2-atp1 and native RPF2 as compared with WT. B) Four-week-old rosettes. C) Six-wk-old plants grown under 18-h photoperiod.