Figure 3.

RPF2-atp1 plants lack Atp1 subunit and assembled respiratory Complex V. A) Separation of crude membrane protein complexes by BN–PAGE of 4 RPF2-atp1 transformants as compared with WT and otp87 (an atp1 editing mutant). The left panel shows the stained membrane after transfer and the right panel the western blot probed with an anti-Atp1 antibody. The black arrows show Complexes I (CI), V (CV), and III (CIII). B) Western blots of mitochondrial proteins of 4 RPF2-atp1 transformants as compared with WT, native RPF2, and otp87 separated by SDS–PAGE.