FIG. 4.

VP22 stabilizes MTs in transfected cells. (A) Untransfected (mock) or transfected (VP22-expressing) COS-1 cells were exposed to different concentrations (ranging from 0 to 5 μg/ml) of nocodazole (noc) for 1 h prior to fixation. The cells were then stained with the anti-α-tubulin monoclonal antibody, and VP22-expressing cells (arrowed) were identified by the presence of MT bundles. (B) Untransfected (mock) or transfected (VP22-expressing) COS-1 cells were incubated at 4°C for 15 min prior to fixation. Staining was carried out as for panel A.