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. 2023 Dec 7;194(4):2117–2135. doi: 10.1093/plphys/kiad629

Figure 1.

Figure 1.

Ovule density and TT are abnormal in the hda19-3 mutant. A) Scheme of the development of the ReT from the CMM from stages 8 to 12 of flower development according to Smyth et al. (1990). Abbreviations: CMM, carpel margin meristem; OP, ovule primordia; mr, medial ridge; TT, transmitting tract. B, C) Cleared pistils of the wild type (B) and hda19-3(C) at stage 9, showing differences in ovule density. Images were digitally extracted for comparison. Scale bar = 50 µm. D) Ovule number of wild-type (n = 70 pistils) and hda19-3 (n = 85 pistils) plants. Center line = median; X = average; box limits = upper and lower quartiles; whiskers = 1.5× interquartile range; points = single measures and outliers. ***Student's t-test with P-value < 0.001. E to H) Transversal section of wild-type (E, G) and hda19-3(F, H) pistils at stage 12. hda19-3 shows unfused septum and defective TT due to the lack of degeneration of septum cells. Images G and H are close-ups of a portion of images E and F, respectively. Abbreviations: TT, transmitting tract; OV, ovule; mr, medial ridge. Scale bar = 50 µm. I) Ratio of pollen tube length/pistil length at 12 and 24 HAP in wild-type (n = 8), heterozygous (n = 8), and homozygous (n = 12) hda19-3 pistils, hand-pollinated with wild-type pollen. Bars represent the mean ± SE value. ***Student's t-test with P-value < 0.001, comparing wild type with HDA19/hda19-3 or hda19-3. J, K) Seed set in the top half and bottom half of wild-type (J) and hda19-3(K) siliques, hand-pollinated with wild-type pollen, showing that the bottom half of hda19-3 siliques shows higher levels of unfertilized ovules than the top half. Bars represent the mean ± SE value of the percentage of unfertilized ovules in 10 wild-type and 10 hda19-3 siliques.