STK regulates STM expression in the CMM. A to H) Detection of STM mRNA in the wild type (A to D) and the stk shp1 shp2 triple mutant (E to H) by ISH. STM is over- and ectopically expressed in the stk shp1 shp2 triple mutant at stages 7 to 10 of pistil development (F, G) as compared to the wild type (B, C). Abbreviations: CMM, carpel margin meristem; LD, lateral domain; OP, ovule primordia; AdR, adaxial replum. Scale bar, 50 µm. I, J) Ovule number (I) and placenta length (J) in wild type (n = 31), stk (n = 35), and stk shp1 shp2 (n = 31). Center dashed line = median; Upper and lower dotted lines = upper and lower quartiles; Violin ends = 1.5× interquartile range; Squares or triangles within the violins = single measures and outliers Letters over violin-plots indicate statistical differences as determined by a one-way ANOVA (P < 0.05) and a Bonferroni post hoc test. K–M) Transversal sections of stages 10 to 11 wild-type (K), hda19-3(L), and stk pistils (M). hda19-3 and stk pistils show defective subepidermal cells in the TT. Abbreviations: TT, transmitting tract; OV, ovule; mr, medial ridge. Scale bar, 50 µm.