Fig. 1. GSDMD promotes inflammation and morbidity following IAV infection.

A-D WT and Gsdmd^−/− mice were intranasally infected with 50 TCID50 of IAV strain PR8. A Western blot of lung lysates taken at day 7 post infection. Each lane represents lysate from an individual mouse. B Weight loss measurements (each dot is an average of individual mouse weights normalized to 100% relative to day 0, error bars indicate SEM, Mocks: n = 4 WT and Gsdmd^−/− from a single experiment, IAV-infected: n = 21 WT, n = 20 KO from 3 independent experiments, p values determined by two-way ANOVA and Bonferroni multiple comparisons test, only statistical comparisons between infected groups are shown). C Survival curve from 3 independent experiments as in B (p value by Log-rank Mantel-Cox test). D Viral titers from lung homogenates from mock-infected, day 3 post infection (from 1 experiment) or day 7 post infection (from 2 independent experiments) (each dot represents an individual mouse, n = 3 mocks, n = 6 day 3 WT and KO, n = 11 for day 7 WT and Gsdmd^−/−, error bars indicate SEM). NS, not significant by two-way ANOVA with Tukey’s Multiple Comparisons Test, only statistical comparisons for WT versus Gsdmd^−/− on days 3 and 7 are shown. Source data are provided in the Source Data file.