Table 2.

Comparison of experimental molecular weights of PARP1-proteins with values calculated from amino acid compositions.

Protein	Measured molecular weighta	Molecular weight from amino acid composition	f/f0	Axial ratio for prolate ellipsoidb
WT-PARP1	109,310 ± 5530	113,907	3.01 ± 0.10	9.92 ± 0.91
∆Zn1∆Zn2-PARP1	90,510 ± 4,610	89,805	2.64 ± 0.09	9.62 ± 0.92
∆CAT- PARP1	73,910 ± 3,590	74,522	2.31 ± 0.09	10.25 ± 0.98

^aMeasured by sedimentation velocity analysis. Error ranges represent 95% confidence limits. ^bCalculated using SEDNTERP⁴¹.