Table 2.

Primer sequences used for mRNA level analysis in the medaka pituitary

Gene	Sequence (5’ - 3’)	Accession Number	PCR product size (bp)	Efficiency	Reference
rpl7	F: TGCTTTGGTGGAGAAAGCTC	NM_001104870	98	2.03	85
	R: TGGCAGGCTTGAAGTTCTTT
gapdh	F: CCTCCATCTTTGATGCTGGT	XM_004077972.3	170	2.01	85
	R: ACGGTTGCTGTAGCCAAACT
lhb	F: CCACTGCCTTACCAAGGACC	NM_001137653.2	100	2	34
	R: AGGAAGCTCAAATGTCTTGTAG
fshb	F: GACGGTGCTACCATGAGGAT	NM_001309017.1	73	2.03	85
	R: TCCCCACTGCAGATCTTTTC
tshba	F: ATGTGGAGAAGCCAGAATGC	XM_004068796.4	88	2	29
	R: CTCATGTTGCTGTCCCTTGA
tshbb	F: CCCAGTGCATTGCTATCAACAC	XM_011477157.2	79	2	This study
	R: CCAAACCGGCCTCTAAAATTGG
mki67	F: ACCAATCTGAGCACAGCCAAC	XM_011487928.3	–	2	99
	R: GGTGCAGGTGGATACTCAAAC
cyp19a1b	F: AAGAAGATGATCCAGCAAGAG	XM_011475856.1	88	2	99
	R: AGCATCAGAAGAAGTAAGAAAAGTG