Fig. 3. NETosis propagation starting from a plaque of stimulated cells.

a Schematic of the NETosis propagation assay. b–e Representative images of NETosis propagation at 20 min (b, c) and 180 min (d) after exposure to stimulated cells. The NETs plaque formed from stimulated cells was pre-stained with Hoechst (blue). The naïve cells (untreated cells) sat at the bottom with the presence of membrane-impermeable DNA stain SYTOX Green (green). Insets: Naïve cells closer to the NETs plaque (d2) showed greater NETosis than cells farther from the NETs plaque (d1).