Fig. 5. NETosis propagation analysis.

a NET Score distributions at different time points. The NET Score was calculated from every 100 × 100 µm² square segment in regions beyond a NETs plaque. Zoomed-in NET Score distributions in selected regions a1 and a2 were shown. b Representative SYTOX Green images of untreated cells from a proximal region (a1) and a distant region (a2). c NET Score averaged over all radial directions at varied radial distances from the plaque edge, at different time points. d NET Score in the radial directions fitted to an inverse exponential after 20 min (nonlinear regression: R² > 0.97).