Table 5.

Effects of increasing substitute alfalfa hay (AH) with hemp forage (HF) in the diet on the ruminal microbiota of Xiangdong black goats at genus level (relative abundance, %).

Phylum	Genus	Treatments1				SEM	P-value2
		CON	HF5	HF10	HF20		T	L	Q
Bacteroidetes	Prevotella_1	29.52a	28.36a	18.81b	19.26b	3.826	0.045	0.040	0.422
	Rikenellaceae_RC9_gut_group	8.28b	9.46b	12.04ab	13.90a	1.649	0.050	0.017	0.746
	Prevotellaceae_UCG-001	2.67	1.13	2.33	2.32	0.853	0.609	0.913	0.508
	Prevotellaceae_UCG-003	1.41	1.89	1.89	1.72	0.442	0.852	0.731	0.451
	Prevotellaceae_NK3B31_group	0.39	1.26	0.39	3.67	1.869	0.568	0.237	0.583
	SP3-e08	0.85	1.81	3.69	2.16	1.274	0.477	0.445	0.220
	Bacteroides	0.69a	0.61ab	0.48bc	0.38c	0.048	<0.01	<0.01	0.628
	Prevotella_9	0.69	0.56	0.36	0.50	0.104	0.195	0.203	0.103
	Paraprevotella	0.49	0.60	0.43	0.52	0.075	0.467	0.895	0.765
Firmicutes	Christensenellaceae_R-7_group	1.85	2.27	1.97	3.49	0.878	0.547	0.205	0.620
	Ruminococcaceae_UCG-002	1.61	3.32	2.06	1.79	0.656	0.274	0.694	0.255
	Ruminococcus_1	1.82	1.07	1.65	1.02	0.360	0.312	0.245	0.931
	Ruminococcaceae_NK4A214_group	0.81b	0.90ab	1.48a	1.04ab	0.154	0.050	0.420	0.167
	Butyrivibrio_2	1.11	1.07	0.55	0.68	0.221	0.225	0.118	0.385
	Ruminococcaceae_UCG-010	0.70	0.65	0.99	0.77	0.122	0.228	0.479	0.248
	Ruminococcaceae_UCG-014	0.82	0.83	0.49	0.55	0.183	0.424	0.206	0.580
	Anaerovorax	0.46	0.54	0.67	0.56	0.057	0.111	0.219	0.104
Fibrobacteres	Fibrobacter	1.34a	0.79ab	1.31a	0.57b	0.297	0.045	0.148	0.703
Spirochaetaceae	Treponema_2	2.09	1.09	1.23	1.07	0.422	0.294	0.176	0.278
Proteobacteria	Ruminobacter	0.40	1.39	2.05	0.40	0.745	0.344	0.889	0.078
	Pseudomonas	0.79	0.68	0.70	0.72	0.042	0.314	0.482	0.151
Unclassified		23.12	24.66	25.68	24.48	4.019	0.847	0.871	0.392
Others		18.14	15.08	18.77	18.44	1.361	0.228	0.447	0.613

^{a, b, c} Means least square of means within a row with different superscripts differ (P ≤ 0.05).

¹CON = 60% AH without HF; HF5 = 55% AH + 5% HF; HF10 = 50% AH + 10% HF; HF20 = 40% AH + 20% HF; AH, alfalfa hay; HF, hemp forage.

²T = treatment; L = linear; Q = quadratic; CON was included in L and Q analysis.