TABLE 1.
Enrichment of pp65 on the nuclear matrix
| Fractiona | % Total cellular proteinb | % of protein in fractionc
|
||
|---|---|---|---|---|
| IE-1 | pp65 (68 kDa) | pp65 (50 kDa) | ||
| Cytoplasm/soluble protein | 86.3 | 93.2 (1.08d) | 5.1 (0.06) | 1.7 (0.02) |
| Detergent-treated nuclei | 8.9 | 2.9 (0.33) | 43.9 (4.93) | 50.2 (5.64) |
| Nuclear matrix | 3.0 | 1.9 (0.63) | 25.9 (8.63) | 38.7 (12.94) |
| Guanidine-HCl-washed nuclear matrix | 1.8 | 2.0 (1.11) | 25.1 (13.94) | 9.4 (5.22) |
HCMV-infected HF were fractionated into the indicated cellular fractions as described in Materials and Methods.
The amount of total protein represented in each fraction was determined as described in Materials and Methods, and the percentage of total cellular protein was calculated as follows: % = (total protein in fraction/total cellular protein) × 100.
The amount of IE-1, pp65 (68 kDa) or pp65 (50 kDa) in each fraction was determined by phosphorimaging. The results are presented as the percentage of total IE-1, pp65 (68 kDa), or pp65 (50 kDa) in each fraction as calculated by the following formula: % = (counts in fraction/total counts for protein) × 100.
The specific enrichment of each protein in a specific fraction, determined by the following formula: enrichment = % of protein determined in footnote c/% of total cellular protein determined in footnote b.