Figure 3.

α5β1 integrin activation mediates Bacopa monnieri extract-induced migration

(A) Immunofluorescence staining for active integrin 12G10 (red) in cells treated with Bacopa monnieri or vehicle control (left panel) and corrected total cellular fluorescence (CTCF; right panel). Scale bar: 10 μm.

(B) Quantification of transwell migration of cells in the presence of vehicle control, Bacopa monnieri extract, RGD peptide, or Bacopa monnieri extract + RGD peptide. p values were calculated using unpaired Student’s t test. ∗p ≤ 0.05, (n = 3 Biological replicate).

(C–E) Quantification of focal adhesion area (C), roundness (D), and number (E) in control (n = 67 cells), Bacopa monnieri-treated (n = 67 cells), RGD peptide-treated (n = 41 cells), or Bacopa monnieri extract + RGD peptide-treated cells (n = 41 cells) in three independent experiments. p values were calculated using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. ∗p ≤ 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.