Figure 4.

Focal adhesion kinase activation mediates Bacopa monnieri extract induced migration

(A) Fluorescence intensity of phospho FAK in human dermal fibroblasts treated with Bacopa monnieri extract or vehicle control.

(B) Quantification of transwell migration of cells in the presence of vehicle control, Bacopa monnieri extract, FAK inhibitor, or Bacopa monnieri extract + FAK inhibitor. p values were calculated using unpaired Student’s t test. ∗p ≤ 0.05, (n = 3 biological replicate).

(C–E) Quantification of focal adhesion area (C), roundness (D), and number (E) in control (n = 41 cells), Bacopa monnieri-treated (n = 67 cells), FAK inhibitor-treated (n = 41 cells), or FAK inhibitor + Bacopa monnieri extract-treated cells (n = 61 cells) in three independent experiments. p values were calculated using one-way ANOVA followed by post hoc Tukey’s multiple comparisons test. ∗p ≤ 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

(F) Model.