TABLE 1.
M6P content of gD molecules expressed by AdgD1 and AdgD1t
| Virus | Protein analyzed | Cell line | % Complexa | % HMa | % 1 M6Pb | % 2 M6Pb | % gDc |
|---|---|---|---|---|---|---|---|
| AdgD1 | gD1 | R970 | 95.4 | 4.6 | 1.9 | 0.9 | 8.4 |
| AdgD1t | gD1t | R970 | 50.3 | 49.7 | 5.5 | 4.6 | 30.3 |
| AdgD1t | gD1t (medium) | R970 | 98.5 | 1.5 | 0.8 | NDd | 2.4 |
a
The percentage of complex or high-mannose (HM) oligosaccharides was determined as described in Materials and Methods based on the assumption that HM oligosaccharides contain an average of 5.1 mannose residues/oligosaccharide and complex oligosaccharides contain three mannose residues/oligosaccharide.
b
1 M6P and 2 M6P, percentage of oligosaccharides bearing one or two M6P residues, respectively.
c
Value obtained by multiplying the total percentage of M6P-modified oligosaccharides by 3, the number of N-linked oligosaccharides per gD molecule.
d
ND, none detected.